High quality RNA extraction from <i>Pseudomonas aeruginosa</i> and other bacteria with a novel rapid and cost-effective method

High-quality mRNA extraction is essential for gene expression assays. In this study, we developed a rapid method (20 min) named FACTS the of intact RNA from Pseudomonas aeruginosa and compared its performance to established techniques like RNAsnap CiAR. The integrity, yield, purity presence residual genomic DNA were adopted as assessment criteria. Multiple assays integrity applied, including Agilent 2200 TapeStation, QIAxcel capillary electrophoresis, newly available Qubit Integrity Quality (IQ) Assay Kit. DNA/RNA yield assessed by spectrophotometry fluorimetry, respectively. Following Dnase treatment, two-step RT-qPCR rpoD reference was performed evaluate each method. terms showed highest while in purity, CiAR scored best. resulted substantial amount DNA. Pilot experiments with other Gram-negative Gram-positive bacteria revealed promising results. novel highly effective high-quality P. can be used cost-efficient alternative methods studies.